Journal: Journal of Neuroinflammation

Article Title: Interleukin-6 and YKL-40 predicted recurrent stroke after ischemic stroke or TIA: analysis of 6 inflammation biomarkers in a prospective cohort study

doi: 10.1186/s12974-022-02467-1

Figure Lengend Snippet: Associations of individual inflammation marker or combination of IL-6 and YKL-40 with recurrent stroke within 1 year

Article Snippet: The concentrations of IL-6, IL-1Ra, Lp-PLA 2 and YKL-40 were determined by using enzyme-linked immunosorbent assay kits (catalogue number: PHS600C for IL-6, PDRA00B for IL-1Ra, DPLG70 for Lp-PLA 2 and DC3L10 for YKL-40, R&D Systems, Inc, Minneapolis, MN, USA).

Techniques: Marker

Journal: Journal of Neuroinflammation

Article Title: Interleukin-6 and YKL-40 predicted recurrent stroke after ischemic stroke or TIA: analysis of 6 inflammation biomarkers in a prospective cohort study

doi: 10.1186/s12974-022-02467-1

Figure Lengend Snippet: Associations of individual inflammation marker or combination of IL-6 and YKL-40 with a Modified Rankin Scale score ≥ 2 within 1 year

Article Snippet: The concentrations of IL-6, IL-1Ra, Lp-PLA 2 and YKL-40 were determined by using enzyme-linked immunosorbent assay kits (catalogue number: PHS600C for IL-6, PDRA00B for IL-1Ra, DPLG70 for Lp-PLA 2 and DC3L10 for YKL-40, R&D Systems, Inc, Minneapolis, MN, USA).

Techniques: Marker, Modification

Journal: Nature Communications

Article Title: Single-cell transcriptomics analysis of bullous pemphigoid unveils immune-stromal crosstalk in type 2 inflammatory disease

doi: 10.1038/s41467-024-50283-3

Figure Lengend Snippet: a Volcano plot of gene features of the CCL19 + FB cluster in BP patients compared to HC. b Volcano plot of gene features of the APCDD1 + FB cluster in BP patients compared to HC. P -values in a and b were obtained using the two-sided Likelihood-ratio test and Bonferroni corrected (Seurat 4). c The feature plot and the bar chart showing PLA2G2A mRNA expression within total skin cells between BP ( n = 5) and HC ( n = 8). d Protein level of PLA2G2A in serum from BP ( n = 73) and HC ( n = 31) examined by ELISA. e Transwell assays were used to measure cell migration of THP1 ( n = 9) and Jurkat ( n = 9) cells treated by PLA2G2A recombinant protein. P -values in c – e were calculated using two-sided Mann–Whitney U-test. * P < 0.05, ** P < 0.01, **** P < 0.0001. In the box plot c – e : Minima: Lower limit of the whisker. Maxima: Upper limit of the whisker. Center: Median line inside the box. The upper and lower box bounds represent the 25% and 75% percentile of data. f Representative images of a BP patient and HC stained by multicolored IHC; green represents PLA2G2A + fibroblasts, red represents macrophages, and yellow represents CD3 T cells. scale bar = 50 μm. Data are from three independent experiments. g Circle plots of the inferred CXCL12 - CXCR4 pathway among major cell types in the BP and HC groups. h Hierarchical plot showing inferred intercellular communication network of CXCL12 - CXCR4 signaling in BP skin. i The PLA2G2A / CXCL12 gene pair co-expression (upper panel), and the gene expression correlation analysis in expressing both genes (lower panel) in fibroblasts. The correlation was measured using the Pearson correlation coefficient. P -values were calculated using two-sided Pearson correlation test. j Flow plots of CD3 + T cells from PBMCs showing the expression of CXCR4 treated by PLA2G2A recombinant protein (upper panel), and the frequency of CXCR4 (lower panel) in BP ( n = 21) and HC ( n = 13) groups. P -values were calculated using paired two-sided Student’s t -test. ** P < 0.01. Each sample is represented as one dot.

Article Snippet: The serum levels of IL-13 (EK0424), IL-4 (EK0404), IL-5 (EK0407), PLA2G2A (EK1944), and CCL17 (EK0684) in both patients and controls were quantified using ELISA Development Kits from BosterBio, USA, following the manufacturer’s instructions.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Migration, Recombinant, MANN-WHITNEY, Whisker Assay, Staining, Gene Expression

Journal: Nature Communications

Article Title: Single-cell transcriptomics analysis of bullous pemphigoid unveils immune-stromal crosstalk in type 2 inflammatory disease

doi: 10.1038/s41467-024-50283-3

Figure Lengend Snippet: a Hierarchical plot showing inferred intercellular communication network of CCL17 - CCR4 signaling. b The feature plot and the bar chart showing CCL17 mRNA expression within total skin cells between BP ( n = 5) and HC ( n = 8). c Protein level of CCL17 in serum from BP ( n = 73) and HC ( n = 32) by ELISA. d The feature plot and the bar chart showing CCR4 mRNA expression within total skin cells between BP ( n = 5) and HC ( n = 8). In the box plot b – d : Minima: Lower limit of the whisker. Maxima: Upper limit of the whisker. Center: Median line inside the box. The upper and lower box bounds represent the 25% and 75% percentile of data. e Flow plots of CD3 + T cells from PBMCs showing the expression of CCR4 treated by CCL17 recombinant protein (left panel), and the frequency of CCR4 (right panel) in BP ( n = 30) and HC ( n = 16) groups. f The positive correlation between the level of PLA2G2A and CCL17 in serum from in BP patients ( n = 73). P -value was calculated using two-sided Pearson correlation test. r -value was Pearson correlation coefficient. g The level of CCL17 in PBMC from BP patients ( n = 26) and HC ( n = 7) treated with PLA2G2A recombinant protein. h Effect of CCL17 treatment on the IL-13 secretion from BP patients ( n = 26) and HC ( n = 13). i Effect of PLA2G2A treatment on the IL-13 secretion from BP patients ( n = 20) and HC ( n = 13). j , k ELISA analysis of anti-BP180-NC16A antibody titers in supernatants of CCL17 ( j ) or PLA2G2A ( k ) stimulated PBMCs from BP patients ( n = 26) and HC ( n = 22). P -values in b–d were calculated using two-sided Mann–Whitney U-test. P -values in e and g – k were calculated using paired two-sided Student’s t -test. * P < 0.05, ** P < 0.01, **** P < 0.0001, only P -values < 0.05 are shown. Each sample is represented as one dot.

Article Snippet: The serum levels of IL-13 (EK0424), IL-4 (EK0404), IL-5 (EK0407), PLA2G2A (EK1944), and CCL17 (EK0684) in both patients and controls were quantified using ELISA Development Kits from BosterBio, USA, following the manufacturer’s instructions.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Whisker Assay, Recombinant, MANN-WHITNEY

Journal: Nature Communications

Article Title: Single-cell transcriptomics analysis of bullous pemphigoid unveils immune-stromal crosstalk in type 2 inflammatory disease

doi: 10.1038/s41467-024-50283-3

Figure Lengend Snippet: It illustrates a positive feedback loop where fibroblasts respond to Th2 cells through the IL13RA1 - IL13 pair, leading to increased secretion of PLA2G2A and CXCL12. This, in turn, amplifies the Th2-mediated response. (i) The lesional fibroblasts respond to IL-13 and induce the overexpression of PLA2G2A, which promotes the expression of CXCR4 on the surface of immune cells to recruit the immune cells from peripheral blood into skin lesions. (ii) Fibroblasts-derived PLA2G2A and myeloid cells-derived CCL17 elevate the secretion of IL-13, and fibroblast and myeloid cells further respond to IL-13, forming a positive feedback loop between immune cells and fibroblasts. (iii) IL-13 activates B cells to secrete autoantibodies. (iv) In blister, the secretion of autoantibodies recruits T cells, myeloid cells, mast cells, neutrophils and eosinophils. T cell-derived IL-13 activates eosinophils to secrete various cytokines (including IL-13), which further promotes Th2 polarization and mediates the crosstalk between immune cells.

Article Snippet: The serum levels of IL-13 (EK0424), IL-4 (EK0404), IL-5 (EK0407), PLA2G2A (EK1944), and CCL17 (EK0684) in both patients and controls were quantified using ELISA Development Kits from BosterBio, USA, following the manufacturer’s instructions.

Techniques: Over Expression, Expressing, Derivative Assay

Journal: Frontiers in Neurology

Article Title: Association Between Plasma Lipoprotein-Associated Phospholipase A2 and Plaque Vulnerability in TIA Patients With Unilateral Middle Cerebral Artery Stenosis

doi: 10.3389/fneur.2020.574036

Figure Lengend Snippet: Characteristics of the studied patients with vulnerable and non-vulnerable plaque.

Article Snippet: Samples for measurement of Lp-PLA2 mass were handled simultaneously by a professional technician using the human Lp-PLA2 enzyme ELISA kit (Hotgen, Beijing, China).

Techniques: Significance Assay

Journal: Frontiers in Neurology

Article Title: Association Between Plasma Lipoprotein-Associated Phospholipase A2 and Plaque Vulnerability in TIA Patients With Unilateral Middle Cerebral Artery Stenosis

doi: 10.3389/fneur.2020.574036

Figure Lengend Snippet: Odds ratios for diagnosis of plaque vulnerability in different models.

Article Snippet: Samples for measurement of Lp-PLA2 mass were handled simultaneously by a professional technician using the human Lp-PLA2 enzyme ELISA kit (Hotgen, Beijing, China).

Techniques: Biomarker Discovery, Significance Assay

Journal: Frontiers in Neurology

Article Title: Association Between Plasma Lipoprotein-Associated Phospholipase A2 and Plaque Vulnerability in TIA Patients With Unilateral Middle Cerebral Artery Stenosis

doi: 10.3389/fneur.2020.574036

Figure Lengend Snippet: Homogeneity determination of lipoprotein-associated phospholipase A2 (Lp-PLA2) across the age strata and interaction of Lp-PLA2 with age.

Article Snippet: Samples for measurement of Lp-PLA2 mass were handled simultaneously by a professional technician using the human Lp-PLA2 enzyme ELISA kit (Hotgen, Beijing, China).

Techniques: Significance Assay

Journal: Frontiers in Neurology

Article Title: Association Between Plasma Lipoprotein-Associated Phospholipase A2 and Plaque Vulnerability in TIA Patients With Unilateral Middle Cerebral Artery Stenosis

doi: 10.3389/fneur.2020.574036

Figure Lengend Snippet: Multivariate logistic regression analysis of variables associated with plaque vulnerability in studied patients aged ≤ 60 years old.

Article Snippet: Samples for measurement of Lp-PLA2 mass were handled simultaneously by a professional technician using the human Lp-PLA2 enzyme ELISA kit (Hotgen, Beijing, China).

Techniques:

Journal: Frontiers in Neurology

Article Title: Association Between Plasma Lipoprotein-Associated Phospholipase A2 and Plaque Vulnerability in TIA Patients With Unilateral Middle Cerebral Artery Stenosis

doi: 10.3389/fneur.2020.574036

Figure Lengend Snippet: Multivariate logistic regression analysis of variables associated with plaque vulnerability in studied patients aged > 60 years old.

Article Snippet: Samples for measurement of Lp-PLA2 mass were handled simultaneously by a professional technician using the human Lp-PLA2 enzyme ELISA kit (Hotgen, Beijing, China).

Techniques: